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Effect of Progesterone: MATERIALS AND METHODS(3)
Experiment 4
White crossbred gilts were unilaterally hysterectomized-ovariecto-mized at approximately 160 days of age, observed once daily for estrous behavior beginning about 200 days of age, and mated by artificial insemination at standing estrus after at least one estrous cycle of normal length. On Day 2 of pregnancy, gilts were assigned at random to receive either 1) corn oil (10 ml) or 2) 400 mg mifepristone in corn oil i.m. At 105 days of gestation, gilts were killed, and the reproductive tract of each gilt was recovered and opened. A blood sample for measurement of hematocrit was collected from each live fetus. The number of live and dead fetuses and the weight of each live fetus were recorded. Brain, heart, and liver weights for each fetus were also recorded. The placenta for each fetus was dissected from the endometrium and weighed.
StatisticalAnalysis
In experiment 1, differences in pregnancy rates between treatments were analyzed using x2 analysis. The number of CL and live fetuses for each gilt were analyzed by ANOVA using a model that included the effect of treatment. Placental weights, fetal weights, and fetal hematocrits were averaged for each litter, and the litter averages were then analyzed using ANOVA with a model that included the effect of treatment.
Tags: embryo estradiol placenta pregnancy progesterone