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Effect of Progesterone: MATERIALS AND METHODS(6)
In experiment 4, pregnancy rates between treatment groups were analyzed using x2. Number of CL, litter size, average fetal hematocrit, average fetal weight, average brain weight, average heart weight, average liver weight, and average placental weight (each trait averaged within litter) were analyzed using ANOVA with a model that included the effect of treatment. Average brain weight, heart weight, and liver weight were also subjected to ANOVA using average fetal weight as a covariate and a model that included the effect of treatment.
The numbers of large (more than the control mean fetal weight) and small (less than the control mean fetal weight) fetuses and the number of large (more than the control mean placental weight) and small (less than the control mean placental weight) placentas were determined for each litter. The effect of mifepristone on the number of large and small fetuses and placentas was analyzed by ANOVA as described for experiment 1. Regression analysis was used to define the relationships between fetal weight and fetal brain, heart, and liver weights. For each trait, the gilt by fetal weight or gilt by fetal weight squared interaction, depending on the order of the relationship (linear or quadratic), was used as the error term.
Tags: embryo estradiol placenta pregnancy progesterone