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As Sertoli cells are difficult to visualize in standard fixation and staining preparations, with only their nuclei being prominent, we utilized an antiprosaposin antibody, a specific marker of Sertoli cells, to stain these cells. Prosaposin has been localized to Sertoli cells, irrespective of the stage of the cycle, and in conjunction with immunocytochemis-try, the antibody readily stains these cells. In this way, we were able to note that Sertoli cells in FORKO mice showed a radial stellate-shaped distribution around each tubule in a manner similar to that seen in wild-type mice. One point that should be noted is that the dilated appearance of the immersed Bouin-fixed Sertoli cells of FORKO mice was not as readily evident as in vascular-perfused, glutaralde-hyde-fixed Epon-embedded tissue. We suspect that the difference is due to the rapid and effective process of crosslinking proteins with glutaraldehyde combined with vascular perfusion that more readily reflects the true status of the tissue. An interesting observation in FORKO mice was the finding that not all seminiferous tubules appeared affected to the same degree, as only about 60% of tubules present in any random section of testis showed the characteristic dilated phenotype in the cytoplasm of Sertoli cells.