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The remaining right testis of each animal was kept intact and immediately fixed by cardiac perfusion with 2.5% or 5% glutaraldehyde buffered in sodium cacodylate (0.1 M) containing 0.05% calcium chloride (pH 7.4). After 10 min of perfusion, the right testes were removed from the scrotum, cut into small 1-mm cubes and placed in the same fixative for an additional 2 h at 4°C. Thereafter, the tissues were thoroughly rinsed three times in 0.1 M sodium cacodylate buffer containing 0.2 M sucrose and left in this buffer overnight. On the following day, the testes were postfixed in ferrocyanide-reduced osmium tetroxide for 1 h at 4°C, dehydrated in a graded series of ethanol and propylene oxide, and embedded in Epon. Thick sections (0.5 |xm) were cut with glass knives and stained with toluidine blue and observed by light microscopy. Thin sections (gray to silver interference color) of selected regions of each block were cut with a diamond knife, placed on copper grids, and counterstained with uranyl acetate (5 min) and lead citrate (2 min). Sections were examined with a Philips 400 electron microscope (Phillips, Eindhoven, The Netherlands). Quantitative Analyses The left testes from the 3- and 6-mo-old wild-type and FORKO mice that had been fixed in Bouin fluid were utilized for quantitative analyses, along with other testes obtained from 12-mo-old wild-type (n = 4) and FORKO (n = 4) mice that had been prepared and fixed in Bouin fluid for a different study.