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LM Immunocytochemistry The following affinity-purified polyclonal antibodies were used at 1: 100 dilution (v/v) for routine peroxidase immunostaining: 1) antiprosa-posin antibody (provided by Dr. C.R. Morales, McGill University, Montreal, Canada; purified and characterized as described previously ) and 2) rabbit anti-ABP antibody prepared against a glutathione sulfo transferase (GST)-ABP fusion protein (provided by Dr. G.L. Hammond, University of Western Ontario, London, Canada). For the antiprosaposin antibody, 5-^m-thick paraffin sections of Bouin fixed testes were deparaffinized in Histoclear (Diamed Lab Supplies Inc., Mississauga, ON, Canada) and hydrated through a series of graded ethanol solutions. During hydration, residual picric acid was neutralized in 70% ethanol containing 1% lithium carbonate, and endogenous peroxidase activity was abolished by treating the sections with 70% ethanol containing 1% (v/v) H2O2. Once hydrated, the sections were washed in distilled water containing glycine to block free aldehyde groups. Nonspecific binding sites were blocked using 10% goat serum for 30 min.