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In the midregion of the epithelium, the dilated spaces often separated chains of round spermatids from each other. In this way, they formed cords or ribbons, which gave the epithelium an anastomotic appearance due to their extensive nature (Fig. 2, a and b). The large dilated spaces of varying sizes also surrounded elongating spermatids, which they often completely enveloped (Figs. 1, b and d, and 2, a and b). The interior of the dilated spaces often contained membranous profiles of varying sizes and spherical particulate material (Fig. 2b). The various generations of germ cells did not display any signs of structural abnormalities or spaces in their cytoplasm (Figs. 1, b and d, and 2, a and b). In the interstitial spaces of FORKO mice that seemed to be enlarged, Leydig cells, macrophages, and blood vessels were structurally similar in appearance to those seen in the wild-type mice. There was no evidence of any increase in macrophage number or infiltration of neutrophils in the interstitial spaces of FORKO mice. Immunocytochemical Analyses Antiprosaposin antibody revealed Sertoli cells spanning the circumference of each tubule in a regular and spokelike manner (Fig. 3, a and b). The staining pattern radiated from the base of the epithelium toward the lumen over a distance that appeared shorter in the FORKO mice due to the smaller profile areas of tubules in these animals compared with wild-type mice (Fig. 3, a and b).